Rabbit monoclonal antibody
Recognizes a protein of 36kDa, identified as Cyclin D1, one of the key cell cycle regulators, is a putative protooncogene overexpressed in 2 wide varieties of human neoplasms. This antibody neutralizes the activity of Cyclin D1 in vivo. About 60% og mantle cell lymphomas (MCL) contain at (11;14)(q13;32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas from CLL/SLL and follicular lymphomas. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
Availability:
| Catalog No. | Contents | Volume |
| ILM3370-C01 | Cyclin D1 | 0,1 ml concentrate |
| ILM3370-C05 | Cyclin D1 | 0,5 ml concentrate |
| ILM3370-C1 | Cyclin D1 | 1,0 ml concentrate |
Intended use: For Research Use Only
Reactivity: Human
Clone: CCND1/3370R
Species of origin: Rabbit
Isotype: IgG
Gene ID: 595
SwissProt: P24385
Unigene: 523852 & 667996
Control Tissue: Human mantle cell lymphoma, breast or bladder carcinomas
Staining: Nuclear
Immunogen: A synthetic peptide corresponding to residues within aa 270-295 of Cyclin D1
Presentation: Bioreactor Concentrate within 0.05% Azide
Application and suggested dilutions:
Pre-treatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0 for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections.
- Immunohistochemical staining of formalin-fixed, paraffin embedded tissue section (dilution 1:50-1:100)
The optimal dilution for a specific application should be determined by the investigator.
Note: Dilution of the antibody in 10% normal goat serum followed by a Goat anti-Rabbit secondary antibody-based detection is recommended.
Storage & Stability: Store at 2-8 °C. Do not use after expiration date printed on the vial.
Reference:
- Baldin V, et al. Cyclin D1 is a nuclear protein required for cell cycle progression in G1. Genes and Development, 1993, 7(5):812-21